rabbit anti human p53 cell signaling  (Cell Signaling Technology Inc)

Journal: Journal of neurochemistry

Article Title: Rotenone affects p53 transcriptional activity and apoptosis via targeting SIRT1 and H3K9 acetylation in SH-SY5Y cells.

doi: 10.1111/jnc.13172

Figure Lengend Snippet: Fig. 5 Neuroprotection of resveratrol in a cellular model of Parkinson’s disease (PD) is SIRT1-dependent. Western blot (a) and quantification (b) of SIRT1 and p53 protein levels after siRNA transfection. (c) qRT- PCR assay to test SIRT1 and p53 changes on mRNA levels. Data are presented as mean SD. *p < 0.05 as compared to mock group (a); **p < 0.01 as compared to mock (a).

Article Snippet: Rotenone and resveratrol affects apoptosis in SH-SY5Y cells To evaluate the neurotoxicity of rotenone in SH-SY5Y cells, we first treated cells with rotenone at various concentrations Rabbit anti-human Bax Cell Signaling Technology WB (1 : 1000) Rabbit anti-human Bcl-2 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human ac-p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human SIRT1 Cell Signaling Technology WB/IP (1 : 1000) Mouse anti-human H3K9-me3 Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human H3K9-ac Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human b-actin Cell Signaling Technology WB (1 : 1000) Rabbit anti-human AMPK Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p-AMPK Cell Signaling Technology WB (1 : 1000) © 2015 International Society for Neurochemistry, J. Neurochem. (2015) 134, 668--676 (0, 0.1, 1, 10, 100 lM) for 24 h, or at 1 lM for different time periods (0, 3, 6, 12, 24, 36 h).

Techniques: Western Blot, Transfection, Quantitative RT-PCR

Journal: Journal of neurochemistry

Article Title: Rotenone affects p53 transcriptional activity and apoptosis via targeting SIRT1 and H3K9 acetylation in SH-SY5Y cells.

doi: 10.1111/jnc.13172

Figure Lengend Snippet: Fig. 7 Model depicting epigenetic regulation of p53 gene expression by SIRT1 in SH-SY5Y cells. (a) The brief structure of P53 gene and the location of H3K9. (b) The relationship of histone methylation and acetylation with gene transcription. (c) The role of SIRT1 in regulating p53 gene expression in SH- SY5Y cells.

Article Snippet: Rotenone and resveratrol affects apoptosis in SH-SY5Y cells To evaluate the neurotoxicity of rotenone in SH-SY5Y cells, we first treated cells with rotenone at various concentrations Rabbit anti-human Bax Cell Signaling Technology WB (1 : 1000) Rabbit anti-human Bcl-2 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human ac-p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human SIRT1 Cell Signaling Technology WB/IP (1 : 1000) Mouse anti-human H3K9-me3 Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human H3K9-ac Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human b-actin Cell Signaling Technology WB (1 : 1000) Rabbit anti-human AMPK Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p-AMPK Cell Signaling Technology WB (1 : 1000) © 2015 International Society for Neurochemistry, J. Neurochem. (2015) 134, 668--676 (0, 0.1, 1, 10, 100 lM) for 24 h, or at 1 lM for different time periods (0, 3, 6, 12, 24, 36 h).

Techniques: Gene Expression, Methylation

Journal: Journal of neurochemistry

Article Title: Rotenone affects p53 transcriptional activity and apoptosis via targeting SIRT1 and H3K9 acetylation in SH-SY5Y cells.

doi: 10.1111/jnc.13172

Figure Lengend Snippet: Fig. 5 Neuroprotection of resveratrol in a cellular model of Parkinson’s disease (PD) is SIRT1-dependent. Western blot (a) and quantification (b) of SIRT1 and p53 protein levels after siRNA transfection. (c) qRT- PCR assay to test SIRT1 and p53 changes on mRNA levels. Data are presented as mean SD. *p < 0.05 as compared to mock group (a); **p < 0.01 as compared to mock (a).

Article Snippet: Rotenone and resveratrol affects apoptosis in SH-SY5Y cells To evaluate the neurotoxicity of rotenone in SH-SY5Y cells, we first treated cells with rotenone at various concentrations Rabbit anti-human Bax Cell Signaling Technology WB (1 : 1000) Rabbit anti-human Bcl-2 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human ac-p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human SIRT1 Cell Signaling Technology WB/IP (1 : 1000) Mouse anti-human H3K9-me3 Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human H3K9-ac Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human b-actin Cell Signaling Technology WB (1 : 1000) Rabbit anti-human AMPK Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p-AMPK Cell Signaling Technology WB (1 : 1000) © 2015 International Society for Neurochemistry, J. Neurochem. (2015) 134, 668--676 (0, 0.1, 1, 10, 100 lM) for 24 h, or at 1 lM for different time periods (0, 3, 6, 12, 24, 36 h).

Techniques: Western Blot, Transfection, Quantitative RT-PCR

Journal: Journal of neurochemistry

Article Title: Rotenone affects p53 transcriptional activity and apoptosis via targeting SIRT1 and H3K9 acetylation in SH-SY5Y cells.

doi: 10.1111/jnc.13172

Figure Lengend Snippet: Fig. 7 Model depicting epigenetic regulation of p53 gene expression by SIRT1 in SH-SY5Y cells. (a) The brief structure of P53 gene and the location of H3K9. (b) The relationship of histone methylation and acetylation with gene transcription. (c) The role of SIRT1 in regulating p53 gene expression in SH- SY5Y cells.

Article Snippet: Rotenone and resveratrol affects apoptosis in SH-SY5Y cells To evaluate the neurotoxicity of rotenone in SH-SY5Y cells, we first treated cells with rotenone at various concentrations Rabbit anti-human Bax Cell Signaling Technology WB (1 : 1000) Rabbit anti-human Bcl-2 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human ac-p53 Cell Signaling Technology WB (1 : 1000) Rabbit anti-human SIRT1 Cell Signaling Technology WB/IP (1 : 1000) Mouse anti-human H3K9-me3 Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human H3K9-ac Cell Signaling Technology ChIP (1 : 1000) Mouse anti-human b-actin Cell Signaling Technology WB (1 : 1000) Rabbit anti-human AMPK Cell Signaling Technology WB (1 : 1000) Rabbit anti-human p-AMPK Cell Signaling Technology WB (1 : 1000) © 2015 International Society for Neurochemistry, J. Neurochem. (2015) 134, 668--676 (0, 0.1, 1, 10, 100 lM) for 24 h, or at 1 lM for different time periods (0, 3, 6, 12, 24, 36 h).

Techniques: Gene Expression, Methylation